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Seba 29. sij
Odgovor korisniku/ci @gsxej @sfrechter i 8 ostali
Many thanks to all authors including Laura Blackie Amy McCarthy Alessio Strano Yoshi Aso
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Seba 29. sij
Odgovor korisniku/ci @sfrechter @as_bates @gsxej
After almost 10y works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
We first validated in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
In one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka !). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
We have developed a new fully genetically encoded tracing system, , and validated it in . This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11
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