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Seba
@SebaCachero
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29. sij |
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Many thanks to all authors including @MarinaGkantia @as_bates @sfrechter Laura Blackie Amy McCarthy @sutcliffe_ben Alessio Strano Yoshi Aso @HHMIJanelia
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29. sij |
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After almost 10y #BAcTrace works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11
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@SebaCachero
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29. sij |
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We first validated #BAcTrace in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11 pic.twitter.com/q56e5gARcD
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@SebaCachero
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29. sij |
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In #BAcTrace one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka #botox!). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11 pic.twitter.com/dn7XZ7SeSW
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Seba
@SebaCachero
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29. sij |
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We have developed a new fully genetically encoded tracing system, #BAcTrace, and validated it in #Drosophila. This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11 pic.twitter.com/p6i4Qav2lI
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