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Michael Desai proslijedio/la je tweet
Nature 20. stu
This week on the Nature cover: Waves of mutation. Barcode system images evolutionary dynamics of laboratory yeast. Browse the issue here:
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @jireva
Over the next couple of days, I'll describe some of the essential components of our technology and how they make long term lineage tracking possible, including: A novel, hyper-directional, set of Lox sites Reiterative Cre-Lox cassette exchange The splicing trap
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @alex_nguyen_ba
and I spent years tracking down rebellious cells that refused to acquire new barcodes. We analyzed how they managed to escape our selections and redesigned our system again and again to try to lead them in the right direction.
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @jireva
The same argument applies to cells that acquire the ability to circumvent selection during gene stacking. The minor flaws of technologies in synthetic biology seem to limit our ability to create complex combinations of simpler constructs.
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @jireva
In a traditional DNA barcoding applications, rare cells that acquire the ability to circumvent barcoding selection are not a big issue. In our case, these rebellious cells would be fatal, since they would have a huge advantage during the next round of barcode addition.
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @jireva
This fusion of these technologies allows us to sequentially add random DNA barcodes into an evolving population to create a time-sorted barcode array at a specific locus in each cell. Earlier barcodes on this array identify more distant ancestors.
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Odgovor korisniku/ci @jireva
Our technique is an attempt to recombine two existing technologies, high resolution DNA barcoding (see ), and gene stacking (e.g. ).
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Michael Desai proslijedio/la je tweet
José Ignacio Rojas Echenique 14. stu
Here's a thread telling the story of our new technique for genetic recording of lineage history during long term evolution experiments. The work was co-led with and in 's lab. Read the paper here:
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Michael Desai proslijedio/la je tweet
Alex N Nguyen Ba 13. stu
What does evolution look like? We developed a rebarcoding approach to observe the travelling wave of adaptation at extremely high resolution. See short summaries by co-authors , in the lab of . Read the paper here:
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Ivana Cvijovic 13. stu
What do you expect evolution to look like in a microbial population? Check out our new study offering a view of that over 1000 generations in yeast in the lab. co-led by , , in 's lab
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Sergey Kryazhimskiy 24. lis
Our Tn-Seq paper with , Alena Martsul and is out in . Accompanied by a nice perspective by Craig Miller with a cool fitness landscape: Here is my original summary:
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Michael Desai proslijedio/la je tweet
Sergey Kryazhimskiy 20. lip
Our new preprint “Higher fitness yeast genotypes are less robust to deleterious mutations” is out. , an amazing PhD student in lab, led this collaborative work. Alena Martsul from my lab also contributed. Please provide feedback!
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Michael Desai proslijedio/la je tweet
Richard Neher 30. pro 2018.
End-of-year rant: Soft sweeps (equivalent adaptations arising independently multiple times) are common in RNA viruses. Stirring up a debate on this non-issue using simplistic models and dubious parameters is a waste of time. More here:
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Michael Desai 6. pro 2018.
Odgovor korisniku/ci @dbweissman @Graham_Coop i 3 ostali
Yep. At least they’re different summary statistics. Maybe.
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Michael Desai 18. stu 2018.
Odgovor korisniku/ci @jmkreinz @Graham_Coop
Just that when graham says linked sel is another form of stochasticity in allele freqs, he’s right. But people often assume that therefore it’s like drift and can be included in variance term in the diffusion eq, and that’s wrong. It’s nondiffusive, has very different signatures
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Michael Desai 14. stu 2018.
Odgovor korisniku/ci @Graham_Coop
The fact that stochasticity from linked sel is fundamentally non diffusive is often not appreciated however, leading to wrong interpretations in terms of Ne
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Michael Desai 8. stu 2018.
Work led by Dan Rice (and in collaboration w/) on signatures of non-Kingman coalescence
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Michael Desai 25. kol 2018.
Odgovor korisniku/ci @jashapiro
Hello!
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Michael Desai proslijedio/la je tweet
Ivana Cvijovic 29. svi 2018.
How does strong background selection influence the site frequency spectrum? Our paper published today online at explains. With and .
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Michael Desai proslijedio/la je tweet
Paul Krugman 31. ožu 2018.
Friday night music on Saturday: from a concert last night
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