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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
Intriguingly, the connection to lateral habenula is potentiated both when tone and footshock are paired or unpaired.
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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
Similarly, SYNPLA works on ex vivo slices of defense conditioned animals. When expressing myc-NRXN in auditory cortex and/or medial geniculate nucleus, we show that paired tone and footshock potentiate the connection to the lateral amygdala relative to controls.
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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
In the paper, we show that SYNPLA works in cultured neurons and hippocampal slice cultures. There we detect a much larger number of SYNPLA signals after chemically induced LTP than in control conditions or when LTP is blocked.
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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
In SYNPLA we probe for endogenous GluA1 and the pathway-specifically expressed pre-synaptic myc-NRXN. We only get a SYNPLA signal if a synapse contains both GluA1 and myc-NRXN . Extra-synaptic GluA1 is too far away from the presynaptic partner to make a signal!
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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
We do this by PLA. PLA is essentially a double antibody stain that results in a 1000x amplified signal only if the two proteins probed for are closer than 40nm to each other. This happens across the synaptic cleft.
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Justus 25. sij
Odgovor korisniku/ci @JustusKebschull
Synaptic GluA1, therefore, is a marker for recently potentiated synapses. The trick is to detect this synaptic GluA1 only in genetically or anatomically specified pathways.
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Justus 25. sij
Odgovor korisniku/ci @TonyZador
So, what is this paper about? SYNPLA is a method to label synapses in a specific pathway that recently underwent LTP. The principle is simple. We exploit the fact that after LTP GluA1 temporarily moves into the synapse from extra-synaptic pools.
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Justus 24. sij
New paper out in PNAS today. Long-running collaboration between Malinow and labs brought to fruition by Kim Dore! Check it out. SYNPLA, a method to identify synapses displaying plasticity after learning
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Jiefu Li 16. sij
Cell-type- and temporally-resolved cell-surface proteomics in intact brains ⁦⁦⁦⁩. Collab. of Liqun's lab, ⁦⁩'s lab, Steve's team ⁦⁩. Thank you all! ⁦⁦⁦⁦
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Tony Zador 23. pro
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Kepecs Lab 5. pro
Thought that frontal cortex is all random mixed selectivity, messy? Think again! Great work by a great team,
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Trends in Neurosciences 1. pro
“Neocortex–Cerebellum Circuits for Cognitive Processing” Review by Mark Wagner & Liqun Luo,
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LMB PhD Programme 28. stu
Neuroscience opportunities in Jing Ren group: Developmental assembly of the serotonin system 🧠😀 Apply NOW Deadline 3 Dec 2019:
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jim mallet 6. stu
We had been rather proud of our 20 genome assembly project in Heliconius, and now we find that Nick Grishin's group has sequenced ALL 845 NORTH AMERICAN BUTTERFLY SPECIES! Mental!
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Jiefu Li 25. lis
Excited to present a method for profiling cell-surface proteomes in intact tissues, with cell-type, spatial, and temporal specificities. A collaboration of the Luo lab, lab, and the proteomics team . Thank you all!
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Alice Ting 25. lis
Proximity labeling in pupae & adult fly brain reveals changes in neuronal surface proteome over development. New synaptic wiring candidates IDed and 20 validated by fly KD. Great collab w/Liqun Luo & Carr lab. Congrats co-1st authors & Shuo Han!
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Justus 21. lis
Are you mapping brain circuits by clearing and light sheet imaging, but have a hard time truely quantifying axonal projections? Check out Drew Friedmann's TrailMap (Luo Lab) now on biorxiv. Then you too can make awesome movies like this one!
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Tony Zador 17. lis
BARseq paper finally out! Connectivity of thousands of neurons per brain, with gene expression, at single neuron resolution, using in situ sequencing.
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Justus 17. lis
Liked this whole sequencing projections idea, but want more spatial resolution? BARseq is now published! De novo in situ sequencing of barcodes + single neuron tracing for thousands of cells at a time, now out from the Zador lab. Read it here:
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Petr Znamenskiy 5. lis
Looking for students and postdocs to join my lab , starting early 2020! Working at the intersection of molecular and systems neuroscience, we’ll study how cell types in visual cortex are wired and contribute to visual perception. Please RT / get in touch if interested!
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