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Justus
@JustusKebschull
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25. sij |
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Intriguingly, the connection to lateral habenula is potentiated both when tone and footshock are paired or unpaired. pic.twitter.com/BktT2SdOB1
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Justus
@JustusKebschull
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25. sij |
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Similarly, SYNPLA works on ex vivo slices of defense conditioned animals. When expressing myc-NRXN in auditory cortex and/or medial geniculate nucleus, we show that paired tone and footshock potentiate the connection to the lateral amygdala relative to controls. pic.twitter.com/3zHp4X1NZx
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Justus
@JustusKebschull
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25. sij |
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In the paper, we show that SYNPLA works in cultured neurons and hippocampal slice cultures. There we detect a much larger number of SYNPLA signals after chemically induced LTP than in control conditions or when LTP is blocked. pic.twitter.com/XxxhnijE2B
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Justus
@JustusKebschull
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25. sij |
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In SYNPLA we probe for endogenous GluA1 and the pathway-specifically expressed pre-synaptic myc-NRXN. We only get a SYNPLA signal if a synapse contains both GluA1 and myc-NRXN . Extra-synaptic GluA1 is too far away from the presynaptic partner to make a signal! pic.twitter.com/lipwzUVRvl
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Justus
@JustusKebschull
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25. sij |
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We do this by PLA. PLA is essentially a double antibody stain that results in a 1000x amplified signal only if the two proteins probed for are closer than 40nm to each other. This happens across the synaptic cleft. pic.twitter.com/BMFl674TVS
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Justus
@JustusKebschull
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25. sij |
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Synaptic GluA1, therefore, is a marker for recently potentiated synapses. The trick is to detect this synaptic GluA1 only in genetically or anatomically specified pathways.
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Justus
@JustusKebschull
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25. sij |
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So, what is this paper about? SYNPLA is a method to label synapses in a specific pathway that recently underwent LTP. The principle is simple. We exploit the fact that after LTP GluA1 temporarily moves into the synapse from extra-synaptic pools.
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Justus
@JustusKebschull
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24. sij |
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New paper out in PNAS today. Long-running collaboration between Malinow and @TonyZador labs brought to fruition by Kim Dore! Check it out.
SYNPLA, a method to identify synapses displaying plasticity after learning pnas.org/content/early/…
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Jiefu Li
@JiefuBiol
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16. sij |
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Cell-type- and temporally-resolved cell-surface proteomics in intact brains @CellCellPress. Collab. of Liqun's lab, @aliceyting's lab, Steve's team @broadinstitute. Thank you all! @Stanford @HHMINEWS cell.com/cell/fulltext/…
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Tony Zador
@TonyZador
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23. pro |
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Ho ho ho, merry BARseq.
@JustusKebschull twitter.com/NIHDirector/st…
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Kepecs Lab
@KepecsLab
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5. pro |
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Thought that frontal cortex is all random mixed selectivity, messy? Think again! Great work by a great team, @ratcortex @agvaughan @PaulMasset @OttTorben twitter.com/gottapatchemal…
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Trends in Neurosciences
@TrendsNeuro
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1. pro |
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“Neocortex–Cerebellum Circuits for Cognitive Processing”
@TrendsNeuro Review by Mark Wagner & Liqun Luo, @Stanford
bit.ly/WagnerLuoTINS
#Cerebellum #Cortex #Reward #MotorControl pic.twitter.com/p3hL5u9wzm
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LMB PhD Programme
@LMB_PhD
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28. stu |
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Neuroscience #LMBPhD opportunities in @MRC_LMB Jing Ren group: Developmental assembly of the serotonin system bit.ly/2oPUoOT 🧠😀
Apply NOW Deadline 3 Dec 2019: bit.ly/2sNTsc3
#PhD pic.twitter.com/1uUmLoStY5
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jim mallet
@WTF_R_species
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6. stu |
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We had been rather proud of our 20 genome assembly project in Heliconius, and now we find that Nick Grishin's group has sequenced ALL 845 NORTH AMERICAN BUTTERFLY SPECIES! Mental! pic.twitter.com/H4PHGIdyVg
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Jiefu Li
@JiefuBiol
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25. lis |
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Excited to present a method for profiling cell-surface proteomes in intact tissues, with cell-type, spatial, and temporal specificities. A collaboration of the Luo lab, @aliceyting lab, and the proteomics team @broadinstitute. Thank you all! twitter.com/biorxiv_neursc…
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Alice Ting
@aliceyting
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25. lis |
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Proximity labeling in pupae & adult fly brain reveals changes in neuronal surface proteome over development. New synaptic wiring candidates IDed and 20 validated by fly KD. Great collab w/Liqun Luo & Carr lab. Congrats co-1st authors @JiefuBiol & Shuo Han!
tinyurl.com/y2ep5qdw
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Justus
@JustusKebschull
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21. lis |
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Are you mapping brain circuits by clearing and light sheet imaging, but have a hard time truely quantifying axonal projections? Check out Drew Friedmann's TrailMap (Luo Lab) now on biorxiv. Then you too can make awesome movies like this one! @_Friedmann_
biorxiv.org/content/10.110… pic.twitter.com/NObM9FJSND
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Tony Zador
@TonyZador
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17. lis |
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BARseq paper finally out! Connectivity of thousands of neurons per brain, with gene expression, at single neuron resolution, using in situ sequencing. @JustusKebschull
sciencedirect.com/science/articl…
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Justus
@JustusKebschull
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17. lis |
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Liked this whole sequencing projections idea, but want more spatial resolution? BARseq is now published! De novo in situ sequencing of barcodes + single neuron tracing for thousands of cells at a time, now out from the Zador lab. @TonyZador
Read it here: tinyurl.com/yxnjc5wa
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Petr Znamenskiy
@petrzzz
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5. lis |
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Looking for students and postdocs to join my lab @TheCrick, starting early 2020! Working at the intersection of molecular and systems neuroscience, we’ll study how cell types in visual cortex are wired and contribute to visual perception. Please RT / get in touch if interested!
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