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Alexander Bates 26. sij
More w/ lab preprints! developed + thoroughly characterised to label neurons inputting those in your favourite genetic lines (opposite to Tango system). Results across many live flies ≈ those from one dead EM’d one
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Greg Jefferis 29. sij
, a : At least since Francis Crick’s famous review of 1979, neuroscientists have dreamed of ways to identify all the neurons connected to a cell of interest. In the last decade this has become a reality through the widespread use of rabies virus tracers. 1/11
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Greg Jefferis 29. sij
Odgovor korisniku/ci @SebaCachero @sfrechter i 5 ostali
We are grateful to long-term support from (to ) and . We will continue to refine the system in flies and we believe that has promise in other organisms including fish and mice. 10/11
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Greg Jefferis 29. sij
Odgovor korisniku/ci @SebaCachero @sfrechter i 2 ostali
Flies will be deposited when the manuscript is accepted. Please contact us if you are interested in obtaining stocks ahead of time. We will ask that you send us feedback on how works for you! 9/11
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Seba 29. sij
Odgovor korisniku/ci @sfrechter @as_bates @gsxej
After almost 10y works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11
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Shahar 29. sij
Odgovor korisniku/ci @as_bates @SebaCachero @gsxej
Although botox is a powerful toxin, we have engineered to be non-toxic to flies (and humans). This allowed us to make whole cell patch clamp recordings from connected neurons for at least 1-2 weeks after BAcTrace labelling. 7/11
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Alexander Bates 29. sij
Odgovor korisniku/ci @SebaCachero @gsxej
We then tried a challenging test. Third-order ‘LHNs’ integrate stereotyped combinations of 5+ input glomeruli. revealed 12/16 connections expected from EM data with a 10 synapse threshold. The top partner had >400 outputs onto the donor cells. 6/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
We first validated in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
In one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka !). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11
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Seba 29. sij
Odgovor korisniku/ci @gsxej
We have developed a new fully genetically encoded tracing system, , and validated it in . This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11
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Bio Products 23. ruj
📌 📌 Estamos a tu orden para información adicional y presupuesto ▶️E-mail: info@bioproducts.net◀️ ▶️📞 ◀️ ®
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Xavier Baudel 29. srp
: Sylvester Stallone second rôle dans un nouveau Direct To Vidéo
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Thomas 13. lip
Qui regarde avec sur C+ ?
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KPL, Inc. 28. pro 2012.
Food Outbreak Sam's Club is Recalling Smoked Salmon for Listeria: via
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