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Alexander Bates
@as_bates
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26. sij |
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More #drosophila #connectomics w/ @gsxej lab preprints! @SebaCachero @MRC_LMB developed + thoroughly characterised #BAcTrace to label neurons inputting those in your favourite genetic lines (opposite to Tango system). Results across many live flies ≈ those from one dead EM’d one twitter.com/fly_papers/sta…
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Greg Jefferis
@gsxej
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29. sij |
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#BAcTrace, a #tweeprint: At least since Francis Crick’s famous review of 1979, neuroscientists have dreamed of ways to identify all the neurons connected to a cell of interest. In the last decade this has become a reality through the widespread use of rabies virus tracers. 1/11 twitter.com/biorxivpreprin… pic.twitter.com/nVGKPvCexH
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Greg Jefferis
@gsxej
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29. sij |
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We are grateful to long-term support from @royalsociety (to @SebaCachero) @MRC_LMB and @ERC_Research. We will continue to refine the system in flies and we believe that #BAcTrace has promise in other organisms including fish and mice. 10/11
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Greg Jefferis
@gsxej
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29. sij |
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Flies will be deposited @flystocks when the manuscript is accepted. Please contact us if you are interested in obtaining stocks ahead of time. We will ask that you send us feedback on how #BAcTrace works for you! 9/11
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Seba
@SebaCachero
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29. sij |
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After almost 10y #BAcTrace works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11
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Shahar
@sfrechter
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29. sij |
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Although botox is a powerful toxin, we have engineered #BAcTrace to be non-toxic to flies (and humans). This allowed us to make whole cell patch clamp recordings from connected neurons for at least 1-2 weeks after BAcTrace labelling. 7/11 pic.twitter.com/5YpvwC3ZkG
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Alexander Bates
@as_bates
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29. sij |
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We then tried a challenging test. Third-order ‘LHNs’ integrate stereotyped combinations of 5+ input glomeruli. #BAcTrace revealed 12/16 connections expected from EM #connectomics data with a 10 synapse threshold. The top partner had >400 outputs onto the donor cells. 6/11 pic.twitter.com/aeSGhvrzLf
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Seba
@SebaCachero
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29. sij |
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We first validated #BAcTrace in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11 pic.twitter.com/q56e5gARcD
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Seba
@SebaCachero
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29. sij |
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In #BAcTrace one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka #botox!). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11 pic.twitter.com/dn7XZ7SeSW
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Seba
@SebaCachero
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29. sij |
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We have developed a new fully genetically encoded tracing system, #BAcTrace, and validated it in #Drosophila. This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11 pic.twitter.com/p6i4Qav2lI
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Bio Products
@BioProductsInc
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23. ruj |
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📌tinyurl.com/y2623t3o 📌
Estamos a tu orden para información adicional y presupuesto
▶️E-mail: info@bioproducts.net◀️
▶️📞 lnkd.in/gJK6NxR ◀️
#KPL #BacTrace® #patógenos #alimentos #perlas #magnéticas #inmunomagnética #DNase #moleculares #latinoamerica #Colombia #bpi pic.twitter.com/35OyGLmKnl
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Xavier Baudel
@hohxb
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29. srp |
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#Bactrace : Sylvester Stallone second rôle dans un nouveau Direct To Vidéo ow.ly/GE9M50veESO
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Thomas
@thomasman3000
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13. lip |
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Qui regarde #Bactrace avec @SlyStallone sur C+ ?
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KPL, Inc.
@KPLBio
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28. pro 2012. |
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Food Outbreak Sam's Club is Recalling Smoked Salmon for Listeria: wp.me/pkt1z-2NV via @wordpressdotcom #BacTrace
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